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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: J Immunol. 2016 Sep 19;197(8):2981–2991. doi: 10.4049/jimmunol.1502045

Figure 3. TLR agonist enhances amount of cryptic peptide.

Figure 3

cDNA constructs encoding [ATG]-YL8 or [CTG]-YL8, along with the Kb MHC Class I molecule and TLR2 were transfected into Cos7 cells. These cells were then treated with Pam3CSK4. Peptides were extracted by acid and fractionated by RP-HPLC. K89 antigen presenting cells and BCZ103 hybridoma cells were added to the fractionated peptide samples to measure the amount of peptide through the T-cell assay. Y-axis indicates the BCZ103 T-cell response and the X-axis indicates the fraction numbers after running the samples through the RP-HPLC. Data is representative of 3 experiments.