Skip to main content
. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: J Immunol. 2016 Sep 19;197(8):2981–2991. doi: 10.4049/jimmunol.1502045

Figure 6. Blocking TNF- and Type I Interferon signaling further inhibits the enhancement of cryptic peptide presentation upon MCMV infection.

Figure 6

A. WI9.LYL8 primary macrophages were infected with MCMV and then cultured with either TNF blocking antibody (MP6XT22), IFNAR 1 blocking antibody along with the respective isotype control antibodies. Data is representative of 2 independent experiments with each experiment having a triplicate of samples for each condition. Cells were then harvested and a T-cell hybridoma assay was set up as described in Figure 2. The Y-axis indicates the T-cell response (11p9Z specific hybridoma response for the conventional peptide and the BCZ103 specific hybridoma response for the cryptic peptide) and X-axis indicates the cell numbers plated. B. T-cell response to infection and blocking antibody, from different experiments, were normalized to that of uninfected samples. The reciprocal of this value was taken and plotted as a % of the uninfected. Mann-Whitney test showed a statistically significant effect of TNF blocking antibody on cryptic peptide presentation.