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. Author manuscript; available in PMC: 2017 Feb 17.
Published in final edited form as: Int J Obes (Lond). 2016 Aug 17;40(11):1627–1634. doi: 10.1038/ijo.2016.142

Figure 2. Validation of HUVEC gene expression patterns identified by array data using Q-PCR.

Figure 2

A. Array expression of top-ranking differentially expressed genes in HUVEC from infants of Ov-Ob vs. lean mothers, including erb-b2 receptor tyrosine kinase 2 (ERBB2), G protein pathway suppressor 1 (GPS1), Kruppel-like factor 7 (KLF7), low density lipoprotein receptor (LDLR), TNFAIP3 interacting protein 2 (TNIP2), and UDP-glucose ceramide glucosyltransferase (UGCG). N=14.

B. qRT-PCR expression of ERBB2, GPS1, KLF7, LDLR, TNIP2, and UGCG, normalized to PPIE and to expression in lean controls. N=20.

C. Based on array analysis, HUVEC expression of protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1) is positively correlated with maternal pre-pregnancy BMI. N=14.

D. qRT-PCR expression of PRKAB1, normalized to PPIE, is positively correlated with maternal pre-pregnancy BMI. N=20. * P<0.05, ** P<0.01, ***P<0.0001.