Figure 3.

Differential Infectivity of Pseudoviruses Supplemented with EBOV Makona GP Mutants in Human Cells

(A–C) Relative infectivity of each glycoprotein was expressed as a proportion (%) of that observed for the Kissidougou-C15 strain in HuH7 (A), BEAS-2B (B), and A549 (C) cells. Histogram bar colors correspond to the lineage color-coding shown in Figure 1, with gray bars indicating variants not sampled during the outbreak. These data are the means ± 1 SD of either two (non-sampled variants) or three (sampled variants) independent experiments, each performed in triplicate. Differences in the mean infectivity of each outbreak-associated mutant compared to the Kissidougou-C15 EBOV strain and the lineage B viruses to the A82V mutant were assessed using repeated-measures one-way ANOVA with Dunnett’s multiple comparison test and indicated in the table inset; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; n.s., not significant.

(D–F) Plots of normalized infectivity for the three human cell lines and correlations were calculated using Pearson’s correlation test. See also Figure S2.