REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Polyethylenimine (PEI)	PolySciences	Cat#23966
Critical Commercial Assays
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	Cat#E0554S
Luciferase Assay Kit	Promega	Cat#1501
GenElute Plasmid Miniprep Kit	Sigma-Aldrich	PLN350
Deposited Data
1610 full-length sequences	Dr. Andrew Rambaut	http://www.virological.org and https://github.com/ebov/space-time
Experimental Models: Cell Lines
Bat kidney cells from Hypsignathus monstrosus	Kuhl et al., 2011	HypNi/1.1
Bat lung cells from Hypsignathus monstrosus	Kuhl et al., 2011	HypLu/45.1
Bat lung cells from Hippsideros abae	Kuhl et al., 2011	HipaLu/24.3
Bat kidney cells from Rousettus aegyptiacus	Kuhl et al., 2011	RoNi/7.1
Bat kidney cells from Epomops buettikoferi	Kuhl et al., 2011	EpoNi/22.1
Bat lung cells from Myotis daubentonii	Kuhl et al., 2011	MyDauLu/47.1
Human embryonic kidney cells	ECACC	HEK293T; RRID: CVCL_0063
Human bronchial epithelial cells	Reddel et al., 1989	BEAS-2B; RRID: CVCL_0168
Human lung alveolus cells	Smith, 1977	A549; RRID: CVCL_0023
Human hepatoma cell line	Nakabayashi et al., 1982	HuH7; RRID: CVCL_0336
Recombinant DNA
GIN_1316_opt_pcDNA3.1(+): Codon optimized version of GenBank: AKG65286.1 in pCDNA3.1 plasmid vector	GenScript	GIN_1316_opt
pTG126 Luciferase encoding plasmid	François-Loïc Cosset	pTG126
phCMV-5349 MLV Gag/Pol-encoding plasmid	François-Loïc Cosset	phCMV-5349
Sequence-Based Reagents
Primers for SDM, see Table S1	N/A	N/A
Software and Algorithms
NEBaseChanger site directed mutagenesis software	New England Biolabs	http://nebasechanger.neb.com/
GTR+I+Γ model in PhyML	Guindon et al., 2010	N/A
PyMOL Molecular Graphics System, Version 1.8	Schrödinger	http://pymol.sourceforge.net; RRID: SCR_000305
PRISM	GraphPad Software	Version 6.05