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Size-Exclusion Chromatography Assays with SDS-PAGE, Related to Figure 2
(A–F) SEC elution profiles of the indicated species were monitored at 495 nm (to follow FAM absorption) and 280 nm (to follow general protein absorption). SDS-PAGE were analyzed for fluorescence (from FAMCENP-C1-71) and also stained with Coomassie Brilliant Blue to visualize all proteins.
