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Brightfield images of three representative MSC spheroids (named as SPa, SPb, and SPc) generated according to the pellet culture method and maintained in low attachment flat-bottom 96-well plates (Non-Tissue Culture Treated Pate, Becton–Dickinson, Franklin Lakes, NJ, USA). After 4 days all the spheroids adhered to the bottom of the plate, thus losing their three-dimensionality
