Fig. 7.

a–c Cell viability analysis. Representative 3D rendering of confocal z-stacks images of spheroids acquired at different time points, showing the spatial distribution of live (green) and dead (red) cells highlighted by Calcein AM and Ethidium Homodimer 1 dyes, respectively. d–f Internal architecture analysis. Representative images of 5 μm cryosections stained with HE and with antibodies against Collagen1A (red in smaller right panels) and Fibronectin (green in smaller right panels). Merge panels show Hoechst nuclear staining overlapped with Collagen1A and Fibronectin. (Color figure online)