Table 2.
Ranking of HKG expression stability across all cell lines grown under serum-free or serum–supplemented conditions calculated using various statistical approaches (in descending order)
| NormFinder stability value* | GeNorm M value⊥ | ||
|---|---|---|---|
| RPLP0 | 0.081 | PPIA | 0.671 |
| PPIA | 0.084 | RPLP0 | 0.674 |
| IPO8 | 0.084 | SDHA | 0.685 |
| YWHAZ | 0.084 | IPO8 | 0.7 |
| RPS23 | 0.086 | GUSB | 0.714 |
| GUSB | 0.088 | RPS23 | 0.725 |
| SDHA | 0.089 | YWHAZ | 0.767 |
| GAPDH | 0.092 | GAPDH | 0.801 |
| MRPL19 | 0.108 | MRPL19 | 0.84 |
| HPRT1 | 0.124 | TBP | 0.873 |
| TBP | 0.126 | HPRT1 | 0.913 |
| B2M | 0.138 | PGK1 | 0.966 |
| PGK1 | 0.138 | B2M | 1.009 |
| UBC | 0.141 | UBC | 1.042 |
| ACTB | 0.150 | ACTB | 1.08 |
| RPLP0 and SDHA | 0.056 | PPIA, RPLP0 and SDHA # | |
Data presented as stability values calculated for each HKG using NormFinder or GeNorm algorithms. A set of genes, the combination of which provides increased stability is presented in the last row (stability value of a set is calculated exclusively by NormFinder)
* Norm Finder stability value is a direct measure for the estimated expression variation. Lower values are indicative of higher expression stability
⊥ GeNorm M value indicates gene expression stability across samples with lower values representing increased stability. Arbitrarily, M values <1.5 are indicative of acceptable expression stability
# The improvement of the GeNorm value is not shown for the combination of PPIA, RPLP0, and SDHA