Table 3.
Line-specific HKG expression stability in cell lines grown under serum-free or serum–supplemented conditions ranked by increasing stability value calculated with NormFinder software
| DLD-1 | HT29 | SW480 | SW620 | HCT116 | Caco-2 | Lovo | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBC | .047 | YWHAZ | .115 | SDHA | .157 | YWHAZ | .062 | B2M | .110 | PPIA | .081 | YWHAZ | .046 |
| PPIA | .061 | B2M | .123 | GUSB | .174 | IPO8 | .097 | YWHAZ | .136 | GAPDH | .108 | GUSB | .050 |
| RPLP0 | .076 | TBP | .142 | YWHAZ | .195 | B2M | .140 | PGK1 | .145 | GUSB | .114 | B2M | .065 |
| RPS23 | .078 | RPLP0 | .169 | RPS23 | .198 | GUSB | .157 | RPLP0 | .147 | B2M | .131 | RPS23 | .076 |
| SDHA | .085 | SDHA | .221 | B2M | .200 | RPLP0 | .158 | UBC | .168 | IPO8 | .137 | HPRT1 | .096 |
| HPRT1 | .093 | IPO8 | .236 | RPLP0 | .210 | HPRT1 | .163 | RPS23 | .186 | RPS23 | .140 | PPIA | .117 |
| YWHAZ | .094 | GAPDH | .258 | GAPDH | .226 | GAPDH | .169 | PPIA | .202 | PGK1 | .153 | UBC | .169 |
| GUSB | .109 | RPS23 | .270 | IPO8 | .256 | RPS23 | .208 | GUSB | .229 | MRPL19 | .156 | ACTB | .172 |
| IPO8 | .123 | PPIA | .274 | PPIA | .256 | PPIA | .210 | HPRT1 | .252 | TBP | .164 | RPLP0 | .197 |
| MRPL19 | .139 | GUSB | .277 | HPRT1 | .268 | SDHA | .212 | SDHA | .262 | RPLP0 | .188 | MRPL19 | .198 |
| GAPDH | .149 | PGK1 | .348 | UBC | .299 | MRPL19 | .283 | GAPDH | .302 | ACTB | .190 | GAPDH | .222 |
| B2M | .155 | ACTB | .357 | PGK1 | .312 | UBC | .304 | MRPL19 | .327 | HPRT1 | .248 | SDHA | .241 |
| PGK1 | .215 | HPRT1 | .362 | TBP | .327 | TBP | .307 | IPO8 | .333 | SDHA | .264 | IPO8 | .243 |
| TBP | .231 | MRPL19 | .383 | MRPL19 | .349 | ACTB | .382 | ACTB | .378 | UBC | .272 | TBP | .258 |
| ACTB | .356 | UBC | .661 | ACTB | .480 | PGK1 | .400 | TBP | .554 | YWHAZ | .309 | PGK1 | .531 |
| PPIA and UBC | .039 | B2M and YWHAZ | .069 | RPS23 and YWHAZ | .098 | IPO8 and YWHAZ | .059 | B2M and PGK1 | .093 | GAPDH and PPIA | .071 | GUSB and YWHAZ | .035 |
Data presented as stability values calculated for each HKG using NormFinder. A set of genes, the combination of which provides increased stability is presented in the last row. Lower values are indicative of higher expression stability