Table 4.
Line-specific HKG expression stability in cell lines grown under serum-free or serum–supplemented conditions ranked by increasing GeNorm M value calculated with qbasePLUS software
| DLD-1 | HT29 | SW480 | SW620 | HCT116 | Caco-2 | Lovo | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| YWHAZ | .005 | YWHAZ | .005 | RPLP0 | .004 | RPS23 | .001 | HPRT1 | .010 | UBC | .010 | B2M | .022 |
| RPLP0 | .005 | GAPDH | .006 | IPO8 | .005 | SDHA | .002 | SDHA | .012 | GUSB | .011 | GUSB | .024 |
| MRPL19 | .007 | TBP | .007 | RPS23 | .006 | IPO8 | .003 | B2M | .015 | RPS23 | .013 | SDHA | .026 |
| PPIA | .011 | RPLP0 | .013 | B2M | .023 | TBP | .010 | TBP | .018 | MRPL19 | .032 | PPIA | .065 |
| B2M | .022 | PGK1 | .025 | UBC | .060 | YWHAZ | .020 | RPLP0 | .020 | B2M | .038 | HPRT1 | .087 |
| HPRT1 | .033 | B2M | .060 | PPIA | .075 | GUSB | .030 | YWHAZ | .030 | IPO8 | .048 | IPO8 | .100 |
| RPS23 | .050 | GUSB | .085 | SDHA | .130 | GAPDH | .050 | MRPL19 | .040 | PPIA | .065 | ACTB | .115 |
| GUSB | .060 | IPO8 | .100 | GAPDH | .180 | PGK1 | .070 | GAPDH | .050 | GAPDH | .080 | MRPL19 | .125 |
| UBC | .068 | PPIA | .110 | GUSB | .205 | ACTB | .090 | GUSB | .062 | YWHAZ | .105 | YWHAZ | .140 |
| IPO8 | .080 | RPS23 | .125 | YWHAZ | .230 | PPIA | .108 | ACTB | .070 | SDHA | .120 | RPS23 | .170 |
| PGK1 | .098 | SDHA | .137 | HPRT1 | .262 | UBC | .123 | UBC | .080 | RPLP0 | .132 | RPLP0 | .190 |
| SDHA | .110 | ACTB | .155 | PGK1 | .285 | RPLP0 | .142 | IPO8 | .122 | HPRT1 | .142 | GAPDH | .210 |
| GAPDH | .130 | MRPL19 | .175 | TBP | .337 | MRPL19 | .160 | PPIA | .170 | TBP | .168 | UBC | .240 |
| TBP | .142 | HPRT1 | .225 | ACTB | .410 | B2M | .218 | RPS23 | .212 | ACTB | .212 | TBP | .270 |
| ACTB | .165 | UBC | .405 | MRPL19 | .460 | HPRT1 | .258 | PGK1 | .262 | PGK1 | .243 | PGK1 | .315 |
| RPLP0 and YWHAZ | GAPDH and YWHAZ | IPO8 and RPLP0 | SDHA and RPS23 | SDHA and HPRT1 | GUSB and UBC | GUSB and B2M | |||||||
Data are presented as stability values (M) calculated for each HKG using GeNorm algorithm. A set of genes, the combination of which provides increased stability is presented in the last row (GeNorm does not provide M value for combination of selected genes). Lower values are indicative of increased stability