Fig. 1.

The effect of sodium butyrate in the presence and absence of 15-Lox-1 inhibitor on breast cancer cell growth. MCF-7 (a) and MDA-MB-468 (b) cells were treated with sodium butyrate (0.5, 1, and 5 mM) for 24, 48, and 72 h. The percentages of viable cells were determined using MTT assay. Cell growth inhibition occurred in a dose and time-dependent manner in both cell lines. The viability of cells at each concentration and time was compared to the viability of control cells in its matched concentration and time. 15-Lox-1 inhibitor attenuated cell growth inhibition induced by sodium butyrate in both MCF-7 (c) and MDA-MB-468 (d) cells. Data represent the mean ± SD of three separate experiments. Statistical differences between treated and untreated groups were analyzed by ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001)