Fig. 5.
Reactive oxygen species (ROS) production in human neurotypic SH-SY5Y cells following treatment with virgin olive oil (VOO) or mixture of hydroxycinnamic acids (Hc acids) caffeic, vanillic, p-coumaric, and ferulic acid. SH-SY5Y cells (2 × 105 cells/mL) were seeded in 96-well plates and were incubated in Opti-MEM with or without Aβ1-42, VOO (1/1000 v/v), or Hc acids mixture for 24 h. The intracellular ROS was determined as described in the Materials and Methods. The intracellular ROS level is proportional to the fluorescence of the standard (DCF) and expressed as percentage (%) of control (untreated). *Statistically significant (P < 0.05) difference between control and treated cells