Fig. 6.
Effect of virgin olive oil or mixture of hydroxycinnamic acids (Hc acids) caffeic, vanillic, p-coumaric, ferulic acid on the expression of activated and non-activated MAPK proteins ERK1/2, p38, and JNK in SH-SY5Y cells. SH-SY5Y cells (2 × 105 cells/mL) were treated with the samples and incubated for 24 h prior to treatment with or without β-amyloid (Aβ)1-42 (control). Protein samples (10 μg) were resolved in 10 % SDS-polyacrylamide gel by polyacrylamide gel electrophoresis, transferred to nitrocellulose membrane, and blotted with primary antibodies from Cell Signaling, Inc. (pERK1/2, ERK2, pJNK, and JNK). The signal was visualized using LiCor Odyssey Infrared Imaging System after reaction with goat anti-mouse IRDye 680LT or goat anti-rabbit IRDye 800CW (LI-COR)