Fig. 3.

Inhibition of DNA-binding NF-κB p65 by vIL-10. a, b lane 1: negative control; a CNE-1 cells were treated with TNF-α alone (lane 2: 0 ng/ml, lane 3: 5 ng/ml, 30 min, lane 4: 100 ng/ml, 30 min); CNE-2 cells were treated with TNF-α alone (lane 5: 0 ng/ml, lane 6: 5 ng/ml, 30 min; lane 7: 100 ng/ml, 30 min). b CNE-1 (lane 2) and CNE-2 (lane 5) were treated without TNF-α or vIL-10; CNE-1 cells were stimulated with TNF-α (15 ng/ml, 30 min) in the presence of vIL-10 (lane 3, 4: 5, 50 ng/ml) as a 30 min pretreatment; CNE-2 cells were stimulated with TNF-α (15 ng/ml, 30 min) in the presence of vIL-10 (lane 6, 7: 5, 50 ng/ml) as a 60 min pretreatment