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. 2016 Oct 26;2016:3087469. doi: 10.1155/2016/3087469

Figure 1.

Figure 1

Apoptosis is inhibited in caspase-3 deficient macrophages and vascular smooth muscle cells. BMDM and VSMCs were isolated from Casp3+/+ApoE−/− (Casp3+/+) and Casp3−/−ApoE−/− (Casp3−/−) mice and treated with cycloheximide (CHX) (0–30 μg/mL) and puromycin (PM) (0–30 μg/mL), respectively. Apoptosis was monitored by (a) western blot analysis for cleaved caspase-3 (β-actin was used as loading control), (b) TUNEL staining (n = 3 independent experiments with 2 counting regions of 200 cells/region in duplicate; ### P < 0.001 and # P < 0.05 versus 0 μg/mL; ∗∗∗ P < 0.001 versus Casp3+/+; factorial ANOVA with genotype and treatment as category factors; Dunnett post hoc) and (c) caspase-3/7 fluorometric activity assay (n = 3 independent experiments; ## P < 0.01, # P < 0.05, and NS, not significant, versus 0 μg/mL; ∗∗∗ P < 0.001 versus Casp3−/−; factorial ANOVA with genotype and treatment as category factors; Dunnett post hoc).