Skip to main content
. 2016 Oct 26;2016:3087469. doi: 10.1155/2016/3087469

Figure 4.

Figure 4

Caspase-3 deficiency in macrophages does not trigger a switch to necroptosis. BMDM were isolated from Casp3+/+ApoE−/− (Casp3+/+) and Casp3−/−ApoE−/− (Casp3−/−) mice and treated with cycloheximide (CHX) (0–30 μg/mL) or oxLDL (25–75 μg/mL) in the presence or absence of the necroptosis inhibitor Necrostatin-1 (Nec-1) (30 μmol/L). BMDM treated with 100 ng/mL LPS and 20 μmol/L zVAD-fmk were used as positive control for necroptosis induction. Necrosis was monitored by PI labeling (n = 3 independent experiments with 2 counting regions of 150 cells/region in duplicate; NS, not significant; ∗∗∗ P < 0.001 versus Casp3+/+ and Casp3−/− without Nec-1; factorial ANOVA with genotype and treatment as category factors; Dunnett post hoc).