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. 2015 Jul 13;13(6):785–794. doi: 10.1038/cmi.2015.72

Figure 2.

Figure 2

Aberrant expression of Il4 and Il17 in IRF8-deficient cells. Splenic naïve CD4 T cells from wild-type or IRF8-deficient mice were differentiated in vitro into Treg for four days. RNA was isolated from the cells and subjected to quantitative RT-PCR. The amount of each gene was normalized to the internal control Hprt (encoding HPRT). The y-axis shows the relative amount between wild-type and IRF8 knockout (KO) (the value of wild type was arbitrarily set 1). The error bar shows standard deviation (n = 3). Statistical difference was analyzed by Student's t-test. *p < 0.05; **p < 0.01; ***p < 0.001. Data are representative of three to five independent experiments with similar results.