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. 2015 Jul 13;13(6):785–794. doi: 10.1038/cmi.2015.72

Figure 3.

Figure 3

Defective expression of CXCR3 in IRF8-deficient Treg cells. (a) Scatterplot of the microarrary analysis. Cxcr3 gene was down-regulated 4.6-fold in IRF8-deficient CD4+CD25+ cells compared that in wild-type counterpart. (b) Splenic CD4+CD25+ cells were isolated from wild-type or IRF8-deficient mice. RNA was isolated from the cells and subjected to quantitative RT-PCR. The error bar shows standard deviation (n = 3). Statistical difference was analyzed by Student's t-test. **p < 0.01. (c) Splenic cells from control (IRF8 fl/fl) or CD4-specifgic IRF8-deficient (IRF8 fl/fl × CD4-Cre) mice were stained with anti-CD4, anti-Foxp3, and anti-CXCR3 antibodies, and analyzed by fluorescence-activated cell sorting (FACS). Cells were gated on CD4.