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. 2016 Nov 9;6:36724. doi: 10.1038/srep36724

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Copyright © 2016, The Author(s)

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The number of unique phyla (A) and genera (B) recovered with culture dependent and culture independent techniques. Numbers recovered are grouped by diesel fuel concentration ranges; low (DL-400 mg kg⁻¹), medium (401–5000 mg kg⁻¹) and high (>5000 mg kg⁻¹). The total extraction of gDNA from SSMS enrichments and directly from soil retrieved considerably more species than the culturing techniques. The pyrosequencing results revealed a peak in unique phyla and genera at mid-level diesel fuel concentrations.