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Expression analysis of sHSPs in chicken embryonic fibroblasts (CEFs) and chicken stage X blastoderm (Stage X) by RT-PCR (a) and quantitative real-time PCR (b,c). HSP25 and HSP30CL were detected specifically in stage X blastoderm (b). Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. Significant differences between groups are indicated as ***P < 0.001. Error bars indicate the SE of triplicate analyses.
