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. 2016 Nov 9;6:36704. doi: 10.1038/srep36704

Figure 3. Multiple alignment and phylogenetic trees of sHSPs containing the α-crystallin domain HSPB9-like.

Figure 3

Multiple sequence alignment (a), phylogenetic trees (b), and similarity to chicken HSP25 (c) of the amino acid sequences of sHSPs containing the α-crystallin domain HSPB9-like. Bold lined amino acids indicate the α-crystallin domain HSPB9-like. Avian HSP25 in red and HSP30CL in blue. Expression analysis of HSP25 and HSP30CL in quail and zebra finch by RT-PCR (d) and during egg storage by qRT-PCR (e). Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. Significant differences between groups are indicated as **P < 0.01. Error bars indicate the SE of triplicate analyses. QEFs, quail embryonic fibroblasts; ZEF, zebra finch embryonic fibroblasts; Blastoderm, blastoderm at oviposition in each species; d, days of storage at 16 °C.