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. 2016 Nov 9;6:36704. doi: 10.1038/srep36704

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Copyright © 2016, The Author(s)

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(a) Knockdown efficiency of HSP25-specific siRNAs in chicken blastoderm cells. Non-complementary sequences in the chicken genome were used as a control. (b) Relative expression analysis of pluripotency marker genes after HSP25 knockdown. Real-time PCR was conducted in triplicate and normalised to the expression of GAPDH. Significant differences between groups are indicated as **P < 0.01 and *P < 0.05. Error bars indicate the SE of triplicate analyses. (c) TUNEL assay performed on chicken blastoderm cells after HSP25 knockdown. Scale bars are 50 μm.