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. 2016 Nov 9;6:36704. doi: 10.1038/srep36704

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Copyright © 2016, The Author(s)

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(a) Annexin V/PI analysis by flow cytometry after HSP25 knockdown followed by mitomycin C treatment (0, 30, or 60 μM). Quantitative analysis of double-negative (b) and double-positive (c) cells, indicating live and apoptotic cells, respectively. (d) Relative expression analysis of anti-apoptotic genes after HSP25 knockdown followed by 60 μM mitomycin C treatment. Non-complementary sequences in the chicken genome were used as a control. Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. ^###P < 0.001 and ^##P < 0.01 significance of mitomycin C treatment (30, or 60 μM) compared to 0 μM. Significant differences between control and HSP25 knockdown are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate the SE of triplicate analyses.