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. 2016 Nov 9;6:36704. doi: 10.1038/srep36704

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) DCFDA analysis by flow cytometry after HSP25 knockdown followed by H₂O₂ treatment (0, 100, or 200 μM). (b) Quantitative analysis of DCFDA-positive cells. (c) Relative expression analysis of anti-oxidant genes after HSP25 knockdown followed by 200 μM H₂O₂ treatment. Non-complementary sequences in the chicken genome were used as a control. Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. Significant differences between control and HSP25 knockdown are indicated as ***P < 0.001 and *P < 0.05. Error bars indicate the SE of triplicate analyses.