Figure 7. Anti-necrotic effect of HSP25 in H₂O₂-treated chicken blastoderm cells.

(a) Annexin V/PI analysis by flow cytometry after HSP25 knockdown followed by H₂O₂ treatment (0, 100, or 200 μM). Quantitative analysis of double-negative (b), double-positive (c) and PI-positive only (d) cells, indicating live, apoptotic, and necrotic cells, respectively. Non-complementary sequences in the chicken genome were used as a control. Significant differences between control and HSP25 knockdown are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate the SE of triplicate analyses.