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. 2016 Nov 9;6:36704. doi: 10.1038/srep36704

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Copyright © 2016, The Author(s)

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(a) Flow cytometry analysis of blastoderm cells with LC3 after HSP25 knockdown followed by MG132 treatment (0, 5, or 10 μM). An Alexa488-conjugated secondary antibody for rabbit IgG was used. (b) Quantitative analysis of LC3-positive cells. (c) Relative expression analysis of pro-autophagic genes after HSP25 knockdown followed by 10 μM MG132 treatment. Non-complementary sequences in the chicken genome were used as a control. Real-time PCR was conducted in triplicate and normalised to expression of GAPDH. ^###P < 0.001 significance of MG132 treatment (5, or 10 μM) compared to 0 μM. Significant differences between control and HSP25 knockdown are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate the SE of triplicate analyses.