Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 24-well Linbro plate |
Temperature (K) | 289 |
Protein concentration (mg ml−1) | 2 |
Buffer composition of protein solution | 10 mM Tris–HCl buffer pH 8.0, 0.1 M NaCl, 0.002% NaN3 |
Composition of reservoir solution | 0.1 M Tris–HCl pH 8.0, 0.2 M sodium formate, 13%(w/v) PEG 4000 |
Volume and ratio of drop | 4 µl (1:1) |
Volume of reservoir (ml) | 0.8 |