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Purity analysis of the JAK1 kinase domain. (a) 4–12% NuPAGE (Invitrogen) SDS denaturing gel. The left lane contains molecular-mass markers (labeled in kDa). (b) Size-exclusion chromatogram of the JAK1 kinase domain. The elution from SEC is later than expected owing to interaction of the protein with the stationary phase of the column.
