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. 2016 Oct 27;72(Pt 11):840–845. doi: 10.1107/S2053230X16016356

Table 1. Expression of the JAK1 kinase domain.

DNA source Human synthetic codon-optimized
Cloning vector pUC57
Expression vector Modified pFastBac
Expression host Sf9
Complete amino-acid sequence of the construct produced MASHHHHHHDYDGATTENLYFQGSIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK