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. 2016 Oct 27;72(Pt 11):840–845. doi: 10.1107/S2053230X16016356

Table 2. Crystallization of the JAK1 kinase domain.

Method Hanging-drop vapor diffusion
Plate type Hampton Research VDXm
Temperature (K) 281
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 25 mM Tris, 250 mM NaCl, 5 mM TCEP, 10 mM ATP, 10 mM MgCl2 pH 8.0
Composition of reservoir solution 30% PEG 1500, 8% MPD, 0.1 M Tris pH 9
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (µl) 500