Table 2. Crystallization of the JAK1 kinase domain.
Method | Hanging-drop vapor diffusion |
Plate type | Hampton Research VDXm |
Temperature (K) | 281 |
Protein concentration (mg ml−1) | 5 |
Buffer composition of protein solution | 25 mM Tris, 250 mM NaCl, 5 mM TCEP, 10 mM ATP, 10 mM MgCl2 pH 8.0 |
Composition of reservoir solution | 30% PEG 1500, 8% MPD, 0.1 M Tris pH 9 |
Volume and ratio of drop | 1 µl:1 µl |
Volume of reservoir (µl) | 500 |