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. 2016 Nov 9;7:397. doi: 10.3389/fphar.2016.00397

Figure 4.

Figure 4

Assessment of Bdkrb1 and Bdkrb2 in the aortas of rat tissues. (A) Immunohistochemistry staining for Bdkrb1 and Bdkrb2 in the aortas of rat tissues. Bdkrb1 protein expression is induced in the aortas of smoking (CS) group. CS-induced expression was abolished in the presence of AO in the (CS + AO). All pictures were taken at 40X magnification. (B) Quantification of the intensity of the immunofluorescence of Bdkrb1. Intensity of Bdkrb1 staining was determined from ZEN software and normalized to the Hoechst level relative to the Control samples. CS group had a significantly higher Bdkrb1 to Hoechst ratio compared to control. Bdkrb1 staining was significantly reduced in CS + AO in comparison with CS. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations (P < 0.05). (C) Quantification of the intensity of the immunofluorescence of Bdkrb2. Intensity of Bdkrb2 staining was determined from ZEN software and normalized to the Hoechst level relative to the Control samples. Bdkrb2 staining was significantly reduced in CS + AO in comparison with CS. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations (P < 0.05). D and E: protein expression assessment of the Bdkrb1 and Bdkrb2 in rat aortas. Protein expression of (D) Bdkrb1, but not (E) Bdkrb2, was induced in the aortas of CS group. AO significantly reduced the expression of Bdkrb1 in the CS + AO group. Data on each protein was normalized to β-actin. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations (P < 0.05).