Table 1. Biological material used in this study.

Jatropha curcas accessions representative of regions in Chiapas State (Mexico) used in this study and their callogenic ability when induced with 2,4-D (5 µM) and BAP (2.5 µM).

Accession*	Latitude	Longitude	Population	Callus dry weight (mg)**
ARR-251107-MFG7	16°11.231′	93°54.516′	Isthmus	1014 ± 578a
MAP-011107-G8	15°25.505′	92°53.554′	Soconusco	402 ± 23ab
JIQ-090208-AG1	16°40.012′	93°39.242′	Center	175 ± 31b
PUJ-030 508-S4	16°16.430′	92°17.550′	Frailesca	204 ± 19b
CDCU-030208-F4	15°40.473′	92°00.129′	Border	207 ± 50b

Notes.

^*Populations located in Chiapas, Mexico. Source: Ovando-Medina et al. (2011).

^**Coefficient of variation was 64.9%. The data were taken 30 days after culture. Each accession had three replicates and each repetition consisted of 20–30 cuttings of a cotyledon. Different letters denote statistical differences revealed by ANOVA and Tukey’s tests (p ≤ 0.05). Composition of medium: Medium MS (1962), 3% sucrose p/v and 500 mg ⋅ L of Polyvinylpyrrolidone + 2,4-D (5 µM) + BAP (2.5 µM).