Table 1. Scoring scheme for quantifying brain damage.
Scoring system for four semi-quantifiable parameters (Cell vacuolation, degenerating neurons, area of cell oedema and area of cell infiltration). Total final score is the combination of A+B+C+D; it is the total of the score assigned for cell vacuolation, neuron degeneration, area of oedema & area of cell infiltration. A High final score indicates higher brain damage. The data obtained from the images with total magnification of 200× were used to determine the final score for each sample.
| Score assigned | Number of cells undergoing vacuolation (A) | Number of degenerating neurons (B) | Percentage of area of oedema (C) (%) | Percentage of area of cell infiltration (D) (%) |
|---|---|---|---|---|
| 0 | 0 | 0 | 0 | 0 |
| 1 | 1–10 | 1–2 | 1–10 | 1–10 |
| 2 | 11–20 | 3–4 | 11–20 | 11–20 |
| 3 | 21–30 | 5–6 | 21–30 | 21–30 |
| 4 | 31–40 | 7–8 | 31–40 | 31–40 |
| 5 | 41–50 | 9–10 | 41–50 | 41–50 |
| 6 | 51–60 | 11–12 | 51–60 | 51–60 |
| 7 | 61–70 | 13–14 | 61–70 | 61–70 |
| 8 | 71–80 | 15–16 | 71–80 | 71–80 |
| 9 | 81–90 | 17–18 | 81–90 | 81–90 |
| 10 | 91–100 | 19–20 | 91–100 | 91–100 |