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. 2016 Apr 4;104(8):1902–1911. doi: 10.1002/jbm.a.35720

Figure 3.

Figure 3

Three dimensional reconstruction images show neuronal differentiation and neurite length without/with FN blocking antibody (FNab) application. Neuronal neurites grow longer with increase in culture days (A–C). Elongation of neurites is not obvious after application of FN blocking antibody (D–F). Statistical analysis reveals that, application of FN blocking antibody does not affect neuronal differentiation of MSCs, but it attenuates neuron‐like cells to elongate their neurites (G). SEM shows neuron‐like cell morphology on 3D gelatin sponge scaffolds. At 14 days after culture, most MSCs‐derived cells with a spindle‐shaped cell body (asterisks in H) protrude thin and long processes perching on the surface of gelatin material (arrows in H). Following FN antibody blocking (asterisks in I), MSCs‐derived cells exhibit a flatter cell body, and shorter and thicker processes (arrows in I). Scale bars: 20 μm in (A–F), (H,I). Asterisk in (G) indicates p < 0.05 (n = 9, Student t‐test).