Fig. 6.

Development of ciliation patterns, nervous system and nephridia in Dinophilus gyrociliatus (depicted with acetylated α-tubulin-LIR). Acetylated α-tubulin-like immunoreactive filaments shown in white, animals are oriented with the anterior end up (b, c-e) or to the left (f-h). Several embryos within one cocoon are shown in a), with the anterior end down to the left (uppermost embryo) or down (lowest embryo to the right). Stages are indicated by silhouettes next to the figure capture, and the assignment to the respective stage next to them. The first signs of difference between the two species D. gyrociliatus and D. taeniatus are emphasized by a yellow dashed-lined frame around the picture. a Female embryos inside the cocoon (3.5–4 days after the eggs were deposited), b late embryos inside the egg layer and cocoon curled with the ventral side outwards showing the ventral nervous system, c detail of b) and the ventral and peripheral nervous system, d ventral view of a prehatching female with the nervous system, nephridia and ciliation pattern, e detail of the head and anterior part of the body, f detail of the ciliary band with cilary band glands, g lateral view of and early juvenile females with nephridia and nervous structures, h lateral view of the nervous and nephridial system in an adult female. Abbreviations: acom – anterior commissure, avcf – anterioventral ciliary field, cb1-8 – ciliary band 1–8, cbg – ciliary band gland, cch – compound cilia of the head, cmvn – circumesophageal commissure forming the medioventral nerve, com1-5 – commissure 1–5, cvlc – circumesophageal commissure forming the ventrolateral nerve cord, fsc – flask shaped cell, lcf – lateral ciliary field, lpn – longitudinal peripheral nerve, mcom – median commissure, mo – mouth opening, mvn – medioventral nerve, n = n1-6 – nephridium 1–6, nacb – lateral nerve anterior to the ciliary band, ncb – nerve of the ciliary band, nis – intersegmental lateral nerve, nlfc – nerve innervating the lateral ciliary field, npcb – lateral nerve posterior to the ciliary band, pcom – posterior commissure, phg – pharyngeal gland, pmvn – paramedioventral nerve, vcf – ventral ciliary field, vlnc – ventrolateral nerve cord