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. 2016 Nov 8;15:543. doi: 10.1186/s12936-016-1596-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Development of the MAL1C-competition ELISA. Optimal dilutions of B-MAL1C and streptavidin-HRP were defined using a checkerboard titration experiment (a). At a 1/16,000 dilution of streptavidin-HRP a sigmoidal curve was observed; this dilution was used for further experiments. B-MAL1C dilutions in the range between the maximal absorbance (plateau observed at 1/50,000) and half max (max/2 observed at 1/400,000) were explored in an inhibition set up using defined sera with high and low antibody content in R32LR ELISA assay (b) and the three monoclonal antibodies, MAL1C, MAL2A and MAL3B (c). The data shown in panels b and c were obtained with the highest serum concentrations (starting dilution 1/5) and with mAb concentrations of 5 µg/ml