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. 2016 Nov 8;16:870. doi: 10.1186/s12885-016-2915-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — M30 staining in untreated for MCF-7 cells had a mean of 1.7 % (a). In cells treated with 60 μg/ml gal-1 for 48 h the rate of very early apoptosis is elevated to up to 6.7 % (p = 0.005, b). The normal rate of apoptosis in MCF-7 breast cancer cells had a mean of 1.4 % detected by in situ nick translation (ISNT, c). The incubation with 60 μg/ml gal-1 for 48 h significantly enhanced apoptosis in MCF7-cells to a maximum of 3.6 % (p = 0.01, d). Results of M30 and ISNT staining are summarized (e) (n = 10)