Table 1.

The kinetic parameters of the wild-type enzyme and the mutants

Parameter	WT	M52-C10	E135V	E135R
K m (mg ml−1)	3.3 ± 0.2	3.0 ± 0.4	2.5 ± 0.6	2.6 ± 0.5
V max (μmol min−1 mg−1)	7178 ± 186	7355 ± 324	8546 ± 654	7734 ± 602
k cat (s−1)	3230 ± 84	3310 ± 146	3846 ± 294	3480 ± 271
k cat /K m (ml mg−1s−1)	978 ± 25	1103 ± 48	1538 ± 118	1339 ± 104