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. 2016 Nov 9;6:36824. doi: 10.1038/srep36824

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Comparison of an epifluorescence image and the reconstructed super-resolution image of tpSil3-Dendra2 with z-focus on the girdle band area of the diatom. The position of the line scan is highlighted. (B) Line scan through the silica cell wall showing the fluorescence intensity profile for both imaging modalities. The FWHM of the PALM image is denoted. (C) Fourier ring correlation for the super-resolution image shown in A reveals an effective resolution estimate of 74.7 nm. Comparison of epifluorescence images and the reconstructed super-resolution image of Dendra2 (D), mEOS3.2 (E) and Dronpa (F) fused to tpSil3 with z-focus on the valve region of the diatom. Enlarged details of the fultoportulae to the right. An SEM image in the same scaling that corresponds to the enlarged detail of the fluorescence image is displayed for comparison. Scale bars are 1 μm, and 100 nm for the zoomed images.