Table 1. Photo-conversion behavior of FPs in diatoms.
FP | Photo-conversion mechanism | Cytosolic expression |
Silica embedded |
Suitable for silica embedded imaging | ||
---|---|---|---|---|---|---|
Before activation | After activation | Before activation | After activation | |||
PATagRFP | PA | Dark | Orange | Dark | Dark | No |
PAmCherry1 | PA | Dark | Orange | Dark | Dark | No |
PA-GFP | PA | Dark | Green | Dark | Dark | No |
mEOS3.2 | PC | Green | Orange | Green | Orange | Yes |
Dendra2 | PC | Green | Orange | Green | Orange | Yes |
Dronpa | PS | Dark | Green | Dark | Green | Yes |
All FPs used for SMLM in diatoms together with the respective screening results are listed. Abbreviations for the conversion mechanism are: photo-activatable (PA), photo-convertible (PC) or photo-switchable (PS). Color names represent the excitation wavelengths 488 nm (green) and 561 nm (orange).