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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Cell. 2016 Aug 11;166(4):950–962. doi: 10.1016/j.cell.2016.07.005

Figure 2. Microtubule methylation during mitosis and cytokinesis in MEFs.

Figure 2

(A) Representative images of cells stained using α-tubulin (red) and α-TubK40me3 (green) antibodies, counterstained with DAPI (blue). The far right panel (Zoom) indicates higher magnification images for better visualization. Scale bar =10μm.

(B) Representative images of cells stained using α-tubulin (red) and H3K36me3 (green) antibodies, counterstained with DAPI (blue). The far right panel (Zoom) indicates higher magnification images for better visualization. Scale bar =10μm.

(C) Quantitative analysis of average fluorescent intensities of midbodies immunostained with H3K36me3 (green curve) and acetylated α-tubulin (red curve) antibodies (N = 66 midbodies, from 3 independent experiments). Representative image showing H3K36me3 (green), acetylated α-tubulin (red) and DAPI (blue). Scale bar =10μm.

See also Figure S2 and S3.