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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Cell. 2016 Aug 11;166(4):950–962. doi: 10.1016/j.cell.2016.07.005

Figure 3. SETD2 methylates lysine 40 of α-tubulin.

Figure 3

(A) Mass spectrometry analysis showing trimethylation at K40 on α-tubulin from HEK-293T cells. Expected molecular weights of trimethylated peptides from the N-terminus and C-terminus are shown as peaks in red and blue, respectively.

(B) In vitro methylation of bovine microtubule proteins using recombinant SETD2 and S-[methyl-3H]-Adenosyl-L-Methionine (SAM) as a methyl donor.

See also Figure S4.