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(a) Filipin-stained normal and NPC1 fibroblasts treated with HP-β-CD (0.01, 0.1, 1 and 10 mM) and HEE-PRXs (0.01, 0.1, and 1 mM of β-CD) for 24 h (scale bars: 50 μm). (b) The amount of total cholesterol in normal and NPC1 fibroblasts treated with HP-β-CD (0.01, 0.1, 1, and 10 mM) and the HEE-PRXs (0.01, 0.1, and 1 mM of β-CD) for 24 h. The data are expressed as the mean ± SD (n = 3) (*p < 0.05, **p < 0.01, ****p < 0.001 vs. untreated NPC1 fibroblasts).
