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. 2016 Sep 26;14(5):4445–4453. doi: 10.3892/mmr.2016.5774

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Copyright: © Tan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Proliferation, cell cycle and migration in T/G HA-VSMCs following various treatments. (A) A sulforhodamine B assay was used to demonstrate the growth of the cells following treatment that compared with siC, siR, siC + P and siR + P at 24, 48, and 72 h,. (B) Cell cycle analysis was performed by flow cytometry following treatment with siC, siR, siC + P and siR + P. The percentage of apoptotic, G0-G1, G2-M or S phase cells were calculated. (C) Microscopy observation (magnification, ×20) of the cells was performed following migration assay. The percentage of cells migrating was calculated. Quantified data are presented as the mean ± standard deviation (**P<0.01 and *P<0.05 compared with the siC group.) si, siRNA; C, control; R, Rab5a; P, PDGF; PDGF, platelet-derived growth factor; T/G HA-VSMC, human aorta-vascular smooth muscle cell line.