Figure 4.

Apoptotic response in T/G HA-VSMCs following various treatments. (A) Flow cytometry was performed to detemin the percentage of cells undergoing apoptosis following treatment with siC, siR, siC + P and siR + P. The data are presented as the mean ± standard deviation (**P<0.01 compared with the siC group.) (B) The protein expression levels of apoptosis-associated proteins, survivin and caspase-3, were determined by western blotting analysis. GAPDH was used as a loading control. si, siRNA; C, control; R, Rab5a; P, PDGF; PDGF, platelet-derived growth factor; T/G HA-VSMC, human aorta-vascular smooth muscle cell line.