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Primer sequences.
| Gene | Primer sequence (5′→3′) |
|---|---|
| Rab5a | |
| Forward | CAGTTCAAACTAGTACTTCTGG |
| Reverse | GCTAGGCTATGGTATCGTTCTTG |
| α-SMA | |
| Forward | GCGTGGCTATTCCTTCGTTA |
| Reverse | ATGAAGGATGGCTGGAACAG |
| Calponin | |
| Forward | AGCTAAGAGAAGGGCGGAAC |
| Reverse | CATCTGCAGGCTGACATTGA |
| Vimentin | |
| Forward | AAAACACCCTGCAATCTTTCAGA |
| Reverse | CACTTTGCGTTCAAGGTCAAGAC |
| Osteopontin | |
| Forward | GGACAGCCGTGGGAAGG |
| Reverse | TCAATCACATCGGAATGCTCA |
| GAPDH | |
| Forward | GCACCGTCAAGGCTGAGAAC |
| Reverse | TGGTGAAGACGCCAGTGGA |
α-SMA, α-smooth muscle actin.
