Table I.
Quantitative profiles of the differentially expressed proteins involved in label-free quantitative proteomics analysis and primer sequences.
| Sequences (5′-3′) | ||||||
|---|---|---|---|---|---|---|
| NCBI number | Protein | Unit peptides | Protein ratio LPS:RTE | Protein ratio rCC16+LPS:LPS | Forward | Reverse |
| gi|6756041 | Tyrosine 3-monooxygenase | 8 | 1.961 | 0.854 | CGAGCGATACGACGAAAT | GCTGATTATTCTCCAGGATGC |
| gi|206440 | Statin-related protein | 5 | 0.540 | 1.384 | GAAGAATGTGTCTGTCAAGGAC | GGGTGGTTCAGGATGATAAC |
| gi|40849846 | UDP glycosyltransferase 1 family polypeptide A8 | 4 | 0.813 | 3.431 | ATGGTCTACATTGGTGGGA | CGCTTTCTTCTCTGGAATCT |
| gi|13928704 | Myosin heavy chain 10, non-muscle | 4 | 5.167 | 0.539 | CAGATTCCTCTCCAACGG | GCAGCACTGAAGACACGA |
| gi|34852966 | Elongation factor 1-α-1 | 3 | 0.533 | 1.617 | TTGTTGCTGCTGGTGTTG | TGGTGACTCAGTGAAATCCA |
| gi|34879484 | Actin-related protein 3 homolog | 4 | 1.517 | 0.648 | GCATCCTGGACCTCAAGA | CAGCGATTGGAATGTGTTT |
| gi|34862480 | Caspase recruitment domain protein 12 | 4 | 0.826 | 1.782 | CCACGGAGGATGAGCAGTA | GGAGGTTCTTCAGATTACCCA |
| gi|34870011 | DNA-dependent protein kinase catalytic subunit | 3 | 0.709 | 1.304 | AGTGTTAGGTGAGGTTCATCCT | CAGTCCCTTTAGACAGCCA |
| gi|34852713 | ATPase, H+-transporting, V1 subunit A, isoform 1 | 1 | 0.793 | 1.368 | GAGAAGCCTCCATTTACACTG | GGTATGCTGGGTATCCACTAA |
| gi|34855075 | Putative adenosylhomocysteinase 3 | 2 | 0.613 | 1.417 | CACTAAGGAATGGCTGGAGTT | CATCAACCTCAGAATCGCA |
| gi|57708 | Acidic ribosomal phosphoprotein P0 | 4 | 1.326 | 0.741 | GACTACACTTTCCCACTGGC | TCCTCTGACTCTTCCTTTGC |
| GAPDH | GTGCCAGCCTCGTCTCATAG | CTTTGTCACAAGAGAAGGCAG | ||||
NCBI, National Center for Biotechnology Information; LPS, lipopolysaccharide; RTE; rat tracheal epithelial cell; CC16, Clara cell protein; UDP, uridine diphosphate; ATPase, adenosine triphosphatase.