Figure 2.

Aldo induced autophagy in MCs. (A) Western blot analysis revealed the protein expression levels of LC3-II/LC3-I, p62, Atg7 and β-actin following treatment with 10⁻⁷ M Aldo for 24 h. (B) Quantification of western blotting revealed the increase in LC3-II/LC3-I and Atg7 protein expression levels, and decrease in p62, following Aldo treatment. Results were normalized to β-actin. Data are presented as the mean ± standard deviation of three series of experiments. ^#P<0.05 vs. control. Aldo, aldosterone; MC, mesangial cell; LC3, microtubule-associated protein 1A/1B-light chain 3; Atg7, autophagy-related gene 7.