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. 2016 Oct 5;14(5):4638–4642. doi: 10.3892/mmr.2016.5807

Figure 4.

Figure 4.

Inhibiting autophagy with siRNA-Atg7 prevented Aldo-induced MC proliferation. (A) MCs were transfected with 20 nM siRNA-Atg7 or siRNA-con as a control. A total of 24 h post-transfection, MCs were treated with 10−7 M Aldo for a further 24 h. Western blot analysis revealed the protein expression levels of LC3-II/LC3-I, Atg7 and β-actin. (B) Quantification of western blotting revealed the decrease in LC3-II/LC3-I and Atg7 protein expression levels following siRNA-Atg7 transfection. Results were normalized to β-actin. Data are presented as the mean ± standard deviation of three series of experiments. (C) MC proliferation was evaluated by [3H]thymidine incorporation following siRNA-Atg7 transfection. Transfection with siRNA-Atg7 abrogated Aldo-induced proliferation of MCs. Data are presented as the mean ± standard deviation of six series of experiments. #P<0.05 vs. control; *P<0.05 vs. Aldo alone. siRNA, small interfering RNA; Atg7, autophagy-related gene 7; Aldo, aldosterone; MC, mesangial cell; con, control; LC3, microtubule-associated protein 1A/1B-light chain 3; con, control.